Table II.

Real-time PCR amplification conditions.

Marker	Pre-denaturation	Denaturation	Annealing	Extension	Cycles
Survivin	95°C (2 min)	95°C (5 sec)	55°C (20 sec)	72°C (20 sec)	40
hTERT	95°C (5 min)	95°C (20 sec)	56°C (30 sec)	72°C (30 sec)	40
CK-7	95°C (2 min)	95°C (15 sec)	58°C (30 sec)	72°C (30 sec)	40
TTF-1	95°C (2 min)	95°C (15 sec)	58°C (30 sec)	72°C (30 sec)	40

Values represent temperature and length of time. hTERT, human telomerase reverse transcriptase; TTF-1, thyroid transcription factor 1; CK-7, cytokeratin-7.