Figure 4.

The number of hilar ectopic DGCs is increased in WT mice with mild and kindled seizures, but not in FGF22KO mice with moderate seizures. Region-matched sagittal hippocampal sections were prepared from PBS- and PTZ-injected WT and FGF22KO mice (3 groups of mice as shown in Figure 1) and stained for Prox1, a marker for DGCs. Ectopic location was defined as greater than or equal to two cell body widths outside the granule cell layer. (A) Prox1 positive cells in the DG and hilus of PBS- or PTZ-injected mice. (B) Quantification of the numbers of ectopic Prox1-positive cells in the hilus. The number of hilar ectopic DGCs was significantly increased in WT mice with mild and kindled seizures, but not in FGF22KO mice with moderate seizures upon PTZ-injections. Bars are mean ± s.e.m. Student's t-test P-values are indicated.