Figure 4.

The effect of CR on SERT expression in adolescent female mouse brain. (a) CR increases the distal polyadenylation fraction (DPF) Brains of mice that had undergone CR (n=4) had elevated levels of SERT mRNA containing the distal polyadenylation sequence than those of control animals (n=4). DPF=relative amount of distal sequence containing SERT mRNA/Total SERT mRNA. (b) CR does not alter the expression of total SERT mRNA. Expression of the coding region of SERT mRNA was compared with a panel of four control genes in the brain from AL mice (n=4) or CR mice (n=4). Data presented is for the average of all four control genes. Differences assessed using Student's t-test. All results are presented as means±SEM. **p⩽0.01.