Table 1. Phenotypes of Venus+ Neurons in the Frontal Cortex.
|
Labeling percentages of the markers expressed in Venus+
cells |
||||
|---|---|---|---|---|
| Period after FLX treatments | 1 week | 2 weeks | 4 weeks | 8 weeks |
| GABA | 39±4.6 (27/70) | 53±5.7 (32/59) | 77±3.9 (34/47)*1 | 84±3.7 (23/29)*1 |
| CR | 19±4.0 (14/72) | 24±3.8 (14/59) | 43±6.2 (19/49)*2 | 44±4.3 (13/28)*3 |
| NPY | 7.3±3.1 (5/66) | 13±4.0 (7/58) | 22±5.7 (10/41)*4 | 27±4.7 (7/25)*5 |
| SOM | 2.5±1.6 (2/69) | 2.1±1.4 (2/73) | 5.8±3.0 (3/44) | 6.1±3.1 (2/24) |
| CB | 0 (0/68) | 0 (0/64) | 0 (0/49) | 0 (0/30) |
| PV | 0 (0/68) | 0 (0/56) | 0 (0/39) | 0 (0/27) |
| S100β | 0 (0/76) | 0 (0/68) | 0 (0/39) | 0 (0/25) |
| Olig2 | 0 (0/80) | 0 (0/66) | 0 (0/40) | 0 (0/27) |
| Iba1 | 0 (0/81) | 0 (0/75) | 0 (0/35) | 0 (0/23) |
Abbreviations: CB, calbindin; CR, calretinin; Iba1, ionized calcium-binding adapter molecule 1; NPY, neuropeptide Y; Olig2, oligodendrocyte transcription factor 2; PV, parvalbumin; SOM, somatostatin; S100β, S100 calcium-binding protein β. Data are presented as the mean values±SEM from 4–5 mice at each time point. The denominator and numerator of the fraction in parentheses represent the total number of Venus+ cells and the number of marker+/Venus+ cells in the frontal cortex, respectively. One-way ANOVA and Scheffé's post hoc test were used in the statistical analysis of the time course of each marker. All P values are vs. 1-week samples for each marker. *1: p<0.001. *2: p=0.0063. *3: p=0.0011. *4: p=0.030. *5: p=0.0037.