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. 2013 May;93(5):751–759. doi: 10.1189/jlb.0912473

Figure 5. Decreased immune complex induced IL-10 by Tlr2−/− macrophages.

Figure 5.

Bone marrow-derived macrophages from WT and Tlr2−/− mice were used to examine FcγR signaling. (A) Representative flow cytometry using in vitro-differentiated macrophages from WT and Tlr2−/− mice demonstrate no difference in the expression of CD11b or F4/80. (B) The FcγR expression on the cell membrane of macrophages (upper-right quadrant gating of A) was determined using an anti-CD16/CD32 antibody, which recognizes FcγRII and FcγRIII. (C) The expression of FcγR isoforms by mRNA using in vitro-differentiated macrophages, was determined by quantitative real-time RT-PCR using primers of FcγRI, FcγRIIb, FcγRIII, FcγRIV, and FcϵRI. (D and E) These in vitro-differentiated macrophages were activated by model immune complexes using wells coated with mouse IgG at concentrations of 10 or 30 μg/ml for 4 h. Macrophage activation was determined by measuring TNF-α (D) and IL-10 (E) in the culture supernatants by ELISA, and the values were normalized for cell number by MTT. Data in C–E are present as the mean ± se of eight mice/group. *P < 0.05 between the indicated groups.