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Anesthesia-induced caspase-3 activation in the IV layer of parietal cortex. Exposure of 5-day-old piglet to the triple anesthesia combination (isoflurane, 0.55-vol% + N2O, 75-vol% + midazolam, 1 mg/kg, i.m.) caused significant caspase-3 activation. Only minimal caspase-3 labeling is shown in sham control (magnification, approximately 100×).
