Fig. 2.

Sub-cellular localisation and dynamics of talin1 and talin2 in various cell types. NIH3T3 cells, smooth muscle cells (SMC) and mouse embryo fibroblasts (MEF) were grown on glass coverslips. (A) Confocal microscopy images of cells stained separately with Mabs to either talin1 (97H6) or talin2 (68E7). (B) Confocal microscopy images of cells co-stained with both talin1 and talin2 Mabs (97H6 and 68E7) followed by isotype-specific secondary antibodies (talin1 97H6 IgG1, talin2 68E7 IgG2b). Talin1 (green); Talin2 (red). (C) NIH3T3 cells stained for talin1 or talin2 either 2 h or 24 h after replating onto glass coverslips. Scale bars = 10 μm. (D) Quantification of number and size of talin1 or talin2 positive structures from (C) 2, 4, 6 or 24 h after replating. All results are expressed as mean ± SEM where N = 30 per time point. P-values for number of talin1 versus talin2 structures per cell: 2 h: 0.0006; 4 h: 0.0001; 6 h: 0.0005; 24 h: 0.089. Size of talin1 versus talin2 structures per cell: 2 h: 0.0001; 4 h: 0.0004; 6 h: 0.074; 24 h: 0.4; *P values of < 0.05. Note: The size of talin2 positive FB at later time points is underestimated due to the “thresholding” method (see the “Materials and methods” section) used to exclude cytoplasmic staining of talin which overlaps talin2 staining in central FB. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of the article.)