Supplementary Figure 2.

(A) In vitro assay of TgSLS with no donor substrate (−) or PI demonstrated that IPC synthase activity was increased approximately 5-fold in the presence of this donor substrate; (B) In contrast, in an equivalent assay ScAUR1 was non-responsive with the bovine PI utilised. AFU – arbitrary fluorescence units. Mean of 3 independent experiments, standard deviation indicated.