Fig. 3.

TgSLS functions as an inositol phosphorylceramide synthase. HPTLC fractionation of lipids after reaction of CHAPS-washed TgSLS extract with acceptor substrate NBD-C₆-ceramide and either no donor substrate (−) or phosphatidylinositol (PI), phosphatidylethanolamine (PE) or phosphatidylcholine (PC). Only the addition of PI led to a significant increase in the product formation, a species migrating with inositol phosphorylceramide (IPC). A representative image, O was the origin, ceramide (Cer) migrated at the front, markers from extracts of NBD-C₆-ceramide labelled yeast (NBD-IPC) and mammalian Vero cells (NBD-SM).