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. 2013 Jan;187(1):43–51. doi: 10.1016/j.molbiopara.2012.11.007

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© 2013 Elsevier B.V.

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Fig. 4 — Inositol phosphorylceramide identification in Toxoplasma gondii lipid extracts. (A) Selected negative ion m/z 778.5234 (inositol phosphorylceramide, N-hexadecanoyl (N-C16) species, IPC); (B) selected negative ion m/z 747.5652 (sphingomyelin, N-hexadecanoyl (N-C16) species, SM); and (C) selected negative ion m/z 659.5128 (ceramide phosphorylethanolamine, N-hexadecanoyl (N-C16) species, CPE) UPLC–TOF chromatograms of mouse embryonic fibroblast (MEF) host cells and T. gondii lipid extracts. (D) Partial mass spectra (from 590 to 850 amu) corresponding to the 6 and 7.8 min range of a representative chromatogram obtained by UPLC/TOF-ESI(−) analysis of lipid extracts of host cells and T. gondii. Regions amplified (20× or 30×) as indicated. (E) Theoretical mass spectral pattern for the molecular ion region showing an (M–H)-ion for CPE and IPC and (M + HCOO⁻) for SM. CPE and SM were found in both samples, whereas IPC was only identified in T. gondii extracts.