Fig. 3.

KSRP^−/− genotype enhances LPS-induced mRNA and protein expression of TNF-α and IL-1β. Left panel: astrocytes were treated with LPS (1 μg/mL) for 24 h, and analyzed for TNF-α and IL-1β mRNAs by qRT-PCR. Target mRNA expression was normalized to S9 mRNA and values are expressed as a fold-induction over unstimulated WT astrocytes (set at a value of 1). Data points are the mean (±SEM) of at least four independent experiments. *P < 0.05 compared with WT. Right panel: ELISA analysis of culture media from astrocytes stimulated with LPS for the time points designated. Data points are the mean (±SEM) of two independent experiments tested in duplicate.