Fig. 4.

LPS-induced TNF-α and IL-1β mRNA stabilization is enhanced in KSRP^−/− astrocytes. WT or KSRP^−/− astrocytes were treated with vehicle (PBS), TNF-α (10 ng/mL) or LPS (1 μg/mL) for 24 h, followed by 5 μg/mL ActD to block transcription. RNA was collected at indicated time intervals and analyzed by qRT-PCR. RNA expression was normalized to S9 and expressed as a percentage of the level before ActD was added (time 0). Values are the mean (±SEM) of at least three independent experiments. Estimated half-lives in hours are shown in parentheses.