Fig. 7.

KSRP specifically inhibits TNF-α and TREM-1 promoter activity in astrocytes. A: Schematic diagram of TNF-α, TREM-1, or Hel-N1 promoters used in the reporter assay. B: Luciferase activity of the reporters after co-transfection with FLAG-KSRP or myc-hnRNP C plasmid at three doses (see Materials and Methods). A transfection control plasmid was used to adjust for transfection efficiency. Values are expressed as a percentage of luciferase activity in cells transfected with reporter alone. Data points for all transfection experiments shown in this figure represent the mean (±SEM) of at least three independent experiments performed in duplicate. *P < 0.03, **P < 0.003, ***P < 0.0003 compared with hnRNP C control at the equivalent gene dose.