Fig. 9.

Conditioned media from KSRP^−/− astrocytes induces cortical neuron toxicity and increased astrocyte migration. A: Rat cortical neurons were treated with different amounts of astrocyte conditioned media (ACM) from KSRP^−/− or littermate control (WT) astrocytes for 24 h. Neurons were stained with a cleaved caspase-3 antibody and DAPI. Immunostained neurons (green) were quantitated. Data points are the mean (±SEM) of eight high-power fields from three independent experiments. **P <0.007; ****P <0.0001 compared with WT. B: WT astrocytes were plated onto tissue culture inserts with 8 μm diameter pores and placed on wells containing KSRP^−/− or control ACM. After 48 h, migrated cells on the lower surface were detected with crystal violet (photomicrographs) and quantified. Data points represent the mean (±SEM) of eight high-powered fields from three independent experiments. ***P < 0.003; ****P <0.0001 compared with WT. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]