Figure 2.

Effect of acute methylphenidate treatment on the uptake of dopamine for untreated and oral methylphenidate treated wild-type flies. The uptake of exogenously applied 1.0 mM dopamine by flies that orally consumed 10 mM methylphenidate paste (black) was compared with flies that did not orally consume methylphenidate (blue). Following baseline dopamine measurements, both groups of flies were treated with bath-applied 1.0 mM methylphenidate for 25 min. There was a significant increase in normalized [DA]_max for the flies that did not receive oral methylphenidate treatment prior to the bath methylphenidate treatment (mean ± SEM; two-way ANOVA, p = 0.05 for interaction, p < 0.0001 for two fly groups, p = 0.03 for bath treatment, n = 5–6).