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Localization of GRA8, GRA9, and SAG2X in in vitro bradyzoite cysts at 4 dpi. BPK1-tagged parasites were grown on HFF monolayers on glass coverslips for 4 days under bradyzoite growth conditions prior to fixation. The images were obtained with antibodies to GRA8, GRA9, and SAG2X (red) or BPK1 tag (venus; green). Representative images from one of at least two experiments are shown. Bars, 10 μm.
