Table 2.
Seroconversion rates after oral infection with in vivo bradyzoite tissue cysts
Strain | Cyst dosea | No. seropositive/totalb | % Seropositivec |
---|---|---|---|
Parental | 100 | 5/15 | 33 |
250 | 4/6 | 67 | |
Δbpk1 | 100 | 1/13 | 8 |
250 | 0/6 | 0 | |
Complemented | 100 | 2/7 | 29 |
250 | 3/7 | 43 |
Cyst dose indicates the number of cysts fed to each mouse. Cysts were enumerated in brain mash by staining with the DBA lectin and counting as described in Materials and Methods.
Number of seropositive mice as determined by Western blotting as described in Materials and Methods shown as a ratio over the total number of mice fed. Pooled data from 6 experiments are shown for the parental and complemented strains. Pooled data from 3 experiments are shown for the complemented strain.
Percentage of mice that seroconverted compared to the total number of mice fed.