Fig 3.

C. albicans Esa1 is essential for hyphal development. (A) Cell morphology of wild-type (BWP17/pBES116) and esa1/esa1 mutant (CWX3/pBES116) cells under liquid conditions. The cells were cultured in liquid YPD medium at 25°C, 30°C, or 35°C for 6 h for yeast growth and in YPD plus 10% serum at 35°C for 3.5 h for hyphal growth. Bars, 5 μm. (B) Colony morphologies of the strains. The cells were plated on solid serum-containing medium and incubated at 35°C for 5 days. (C) Invasive growth on agar. The strains were patched on YPD plates and grown at 35°C for 5 days. After washing with water, the agar containing cells was cut into slices for photographing. (D) Filamentous growth under embedded conditions. The cells were plated with molten YPS agar and grown at 25°C for 3 days or 35°C for 1 day.