Fig 4.

Effects of C. albicans Esa1 on gene regulation. (A) Quantitative RT-PCR analyses showed defective induction of hypha-specific genes HWP1 and ECE1 in esa1/esa1 mutants. Cells were grown in YPD plus 10% serum at 35°C for 3.5 h or grown in YPD at 25°C, 30°C, or 35°C for 6 h. (B) Expression levels of heat shock genes HSP90, HSP70, and HSP104 were inhibited in esa1/esa1 mutants. Cells were grown in YPD at 25°C and then heat shocked at 35°C for 0.5 hour. (C) Expression levels of stress response genes HSP12, TPS1, and TPS2 were derepressed in esa1/esa1 mutants. Cells were cultured in YPD at 25°C and then upshifted to 35°C or downshifted to 4°C for 0.5 hour, and total RNAs were extracted for qRT-PCR analyses.