Fig 5.

C. albicans Esa1 is involved in multiple stress responses. (A) esa1/esa1 mutants are sensitive to genotoxic and oxidative stresses. Wild-type (BWP17/pBES116) and esa1/esa1 mutant (CWX3/pBES116) cells were serially diluted 10-fold, spotted onto YPD plates containing DNA-damaging agents (10 mM HU, 0.01% MMS, and 15 μg/ml CPT) or 2 mM H₂O₂, and incubated at room temperature (rt; 22°C) for 3 days. (B and C) esa1/esa1 mutants were resistant to osmotic and cell wall stresses. YPD plates containing 5 M NaCl, 100 μg/ml Congo red, or 0.32 μg/ml caspofungin (B), or YP with 5 M NaCl or 10% sucrose (C) were used for growth assays. SC plates containing 0.1% glucose (SCLD) and low ammonia (SLAD) were used for a nutrient-sensing assay. (D and E) YP plates (D) or SC plates (E) containing 2% glucose, maltose, ethanol, or glycerol were used for a carbon source-sensing assay.