Fig 6.

Candida albicans open reading frame 19.2087 encodes MYST protein Sas2, which plays a role opposite to that of Esa1 in cell growth and morphogenesis. (A) Schematic depiction of the MYST domain and chromo domain in C. albicans Sas2, S. cerevisiae Sas2, and human MOF. (B) sas2/sas2 mutant cells were tolerant to high temperature. The cells were spotted onto YPD plates and incubated at 25°C, 37°C, 42°C, or 45°C for 2 days. (C) Sas2 and Esa1 have opposite effects on filament formation. Wild-type (BWP17/pBA1), wild-type with ADH1p-ESA1 (BWP17/pBA1-ESA1), esa1/esa1 mutant (CWX3/pBA1), and sas2/sas2 mutant (CWX6/pBA1) cells were grown in liquid YPD medium with or without 10% serum at different temperatures for 3 h. −, yeast growth; +, filamentous growth. The level of cell elongation is indicated by the number of + symbols. N/A, not available (cells failed to grow).