Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| SC5314 | Wild type | 24 |
| CAI4 | ura3::λ imm434/ura3::λ imm434 | 24 |
| BWP17 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG | 23 |
| CWX1 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG ESA1/esa1::HIS1 | This study |
| CWX2 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG esa1::HIS1/esa1::hisG-URA3-hisG | This study |
| CWX3 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG esa1::HIS1/esa1::hisG | This study |
| CWX4 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG SAS2/sas2::HIS1 | This study |
| CWX5 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG sas2::HIS1/sas2::hisG-URA3-hisG | This study |
| CWX6 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG sas2::HIS1/sas2::hisG | This study |
| CWX7 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG esa1::ARG4 Ptet-ESA1::URA3 | This study |
| CWX8 | ura3::λ imm434/ura3::λ imm434 his1::hisG/his1::hisG arg4::hisG/arg4::hisG sas2::HIS1/sas2::hisG-ura3-hisG esa1::ARG4 Ptet-ESA1::URA3 | This study |
| Plasmids | ||
| pCUB6 | Replacement of S. cerevisiae URA3 by C. albicans URA3 in pNKY50 | 24 |
| pESA1-KO | 0.53- and 0.6-kb KO fragments of C. albicans ESA1 and hisG-URA3-hisG in pBluescript SK(+) | This study |
| pSAS2-KO | 0.55- and 0.5-kb KO fragments of C. albicans SAS2 and hisG-URA3-hisG in pBluescript SK(+) | This study |
| pBES116 | C. albicans URA3 vector for integration at ADE2 locus | 53 |
| pBES116-ESA1 | 2.18-kb CaESA1 in pBES116 for ESA1 expression under control of ESA1 promoter | This study |
| pBA1 | C. albicans ADH1 promoter in BES116 | 25 |
| pBA1-WX1 (pBA1-ESA1) | 1.56-kb full-length CaESA1 in pBA1 for ESA1 expression under control of ADH1 promoter | This study |
| pBA1-WX2 | 1.2 kb of CaESA1 with deletion of chromo domain in pBA1 | This study |
| pBA1-WX3 | 0.9 kb of CaESA1 with deletion of HAT domain in pBA1 | This study |
| pBA1-WX4 | 1.3 kb of CaESA1 with deletion of insertion sequence in pBA1 | This study |
| pBA1-WX5 | 1.45-kb full-length ScESA1 in pBA1 | This study |
| pBA1-WX6 | ScESA1 G315E mutation in pBA1 | This study |
| pBA1-WX7 | ScESA1 L327S mutation in pBA1 | This study |
| pBA1-WX8 | 1.45-kb full-length human TIP60 cDNA in pBA1 | This study |
| pBA1-WX9 | Hybrid of CaEsa1 chromo domain and hTIP60 MYST domain | This study |
| pBA1-WX10 | Hybrid of hTIP60 chromo domain and CaEsa1 MYST domain | This study |
| p671 | C. albicans ACT1 promoter, 13×myc-FLAG1, HIS1 | 25 |
| p671-ARG4 | HIS1-to-ARG4 substitution in p671 | This study |
| pCaUME6-3 | Supplies ADH1p-cartTA-GAL4AD fragment | 54 |
| pCPC39 | Supplies tet-on promoter and URA3 marker | This study |
| pCPC42 | Supplies tet-off promoter and URA3 marker | This study |
| pCPC97 | Infusion of pUC18 and fragment containing CaESA1 (bp −521 to +1979) | This study |
| pCPC98 (pPtet-ESA1) | Infusion of pCPC97 and pCPC42 for doxycycline-controlled ESA1 expression | This study |