Fig 1.

Comparative antimicrobial peptide susceptibilities and time courses of RTA2 and BCR1 mRNA levels in two C. albicans bloodstream isolates (CA024 [Amp^s] and CA080 [Amp^r]) with differing levels of antimicrobial peptide susceptibility and in the DAY185 reference strain. (A) Susceptibilities of the three C. albicans strains to the indicated antimicrobial peptides were determined by a radial diffusion assay at pH 7.5. Antimicrobial susceptibility was measured as the zone of inhibition (ZOI) after incubation at 30°C for 24 h. Results are means ± standard deviations (SD) from three independent experiments. (B and C) RTA2 (B) and BCR1 (C) transcript levels in the Amp^s, Amp^r, and DAY185 strains after incubation for the indicated time in the presence of a sublethal concentration of RP-1 (2.5 μg/ml for Amp^s, 100 μg/ml for Amp^r, and 5 μg/ml for DAY185). Transcript levels were measured by real-time PCR using ACT1 as the endogenous control gene and normalized to organisms incubated for 60 min in medium without RP-1 (untreated). Results are means ± SD for three biological replicates, each measured in duplicate. *, P < 0.05 compared to cells grown in the absence of RP-1. HNP-1, human neutrophil peptide 1; hBD-2, human β-defensin 2.