Fig 6.
Effects of RP-1 on C. albicans plasma membrane permeability and mitochondrial membrane potential. The indicated strains of C. albicans were exposed to RP-1 at pH 7.5 for 1 h and then analyzed by flow cytometry. (A and C) Histograms of propidium iodide fluorescence, a measure of membrane permeabilization, of the Amps and Ampr strains exposed to 5 μg/ml RP-1 (A) and of strain DAY185 exposed to 5 to 20 μg/ml RP-1 (C). The fluorescence of untreated control cells is indicated by the black lines, and the fluorescence of cells exposed to RP-1 is indicated by the red lines. (B and D) Histogram of DiOC5 fluorescence, a measure of mitochondrial membrane potential, of the Amps and Ampr strains exposed to 5 μg/ml RP-1 (B) and of strain DAY185 exposed to 5 to 20 μg/ml RP-1 (D). The fluorescence of untreated control cells is indicated by the black lines, and the fluorescence of cells exposed to RP-1 is indicated by the green lines.