FIG. 1.

Sensitive and robust responses to pre-analytical conditions. (A) The concentrations of three analytes (GGT, LDH, and glucose) in serum showed significant differences after 2 h that were dependent on the duration of delay before centrifugation. In contrast, the concentrations of TG, TC, and creatinine did not change. (B) Responses of GGT, LDH, and BUN were sensitive to the duration of delay after fractionation. (C) Levels of GGT, LDH, and BUN in plasma changed significantly with the number of freeze-thaw cycles. However, the levels of TC, creatinine, and glucose did not change after nine freeze-thaw cycles.