TABLE 2.
The effects of amino acid mutations of recombinant hCB1 receptors on the displacement of [3H]SR141716A by CP55,940
Data represent the mean and corresponding 95% confidence limits of at least three independent experiments performed in triplicate. The Ki value of CP55,940 at the mutant receptors was not statistically significantly different from wild-type CB1 receptors using a two-tailed Student’s t test.
| [3H]SR141716A | CP 55,940 (Ki) + 95% CL |
|---|---|
| WT | 17 nM (5.3–53) |
| D2.63176A | 4.9 nM (0.6–43) |
| K373A | 17 nM (3.3–85) |
| D2.63176A-K373A | 5.1 nM (0.6–42) |
| D2.63176K-K373D | 15 nM (3.0–69) |