Figure 2. TXNIP is required for activation of VEGFR2 and PLCγ1 in EC.
(A) HUVEC were transfected with control siRNA or TXNIP siRNA. After 48 hrs, the cells were treated with VEGF (10 ng/ml) for the indicated times. Cell lysates were immunoblotted with p-VEGFR2 (Y1175), VEGFR2, p-PLCγ1 (Y783), and PLCγ1. (B-C) Lower panels are quantification analysis. *P<0.05 vs. control siRNA+VEGF (mean ± SD; n=3).
