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. Author manuscript; available in PMC: 2014 Mar 20.
Published in final edited form as: Neuron. 2013 Mar 20;77(6):1151–1162. doi: 10.1016/j.neuron.2013.01.038

Figure 1. Expression of hM4D in the medio-dorsal thalamus using viral vector.

Figure 1

(A) Schematic drawing of pAAV2-hM4D-IRES-hrGFP plasmid. Both hM4D (red) and hrGFP (green) are expressed under the control of a cytomegalovirus (CMV) promoter (yellow). (B) Native hrGFP fluorescence (green) and anti-HA immunostaining (red) reveal co-expression (yellow) of GFP and hM4D in MD cells. (C) Top panel: native hrGFP and anti-NeuN immunostaining (red) reveal that hrGFP is exclusively expressed in neurons. Bottom panel: stereotactic injection of the AAV2-hM4D-IRES-hrGFP leads to transgene expression in 27 ±6% of MD neurons with a peak at 66 ±9.6% at the site of infection (n=4). (D) GFP labels the MD projections to the PFC after injection with a hrGFP control virus in the MD. Abbreviations: prelimbic (PrL), medial ventral orbital (VO), lateral orbital (LO) dorsolateral orbital (DLO) cortices, I, III/V depict cortical layers. See also figure S1.