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. Author manuscript; available in PMC: 2014 Mar 20.
Published in final edited form as: Neuron. 2013 Mar 20;77(6):1151–1162. doi: 10.1016/j.neuron.2013.01.038

Figure 2. Decreasing activity in the medio-dorsal thalamus using a pharmaco-genetic approach.

Figure 2

(A) Left panel: visualization of hM4D ires hrGFP expressing neurons in a thalamic slice. Right panel: Resting membrane potential of MD neurons infected with control hrGFP (green) and experimental hM4D ires hrGFP (orange) virus before and after bath application of 1 μM CNO (repeated measures ANOVA, group x treatment interaction ***p<0.001; n=11 and 10 cells for hrGFP and hM4D-hrGFP cells). (B) Example of MD unit activity in vivo before (green) and after (orange) systemic injection of 2 mg/kg CNO to anMDhM4D mouse. (C) Histogram of the ratios of firing rate after CNO to firing rate after saline in MDhM4D mice. Red and blue bars represent cells with statistically significant increases or decreases in firing rate, respectively. Inset: histogram of the ratios of firing rate after CNO to firing rate after saline in wild-type mice. (D) Firing rate after saline vs. firing rate after CNO for entire sample of 63 MD units. Red and blue circles indicate units with statistically significant increases or decreases in firing rate, respectively (p<0.05, paired t-test). Inset is a magnified view of lower firing-rate neurons. Error bars: s.e.m.See also figure S2.