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. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: Nature. 2013 Feb 17;495(7439):111–115. doi: 10.1038/nature11833

Figure 2.

Figure 2

FRQ activities in circadian feedback loops are impaired in the frq codon-optimized strains. (a) Immunoprecipitation assay showing that FRQ has a decreased ability to interact with WC-2 in the codon-optimized strains. Densitometric analyses of results from four independent experiments are shown below. PI: pre-immune serum; IP: immunoprecipitation. Error bars indicate standard error (n=9). Asterisks indicate the p value <0.01. (b and c) Western blots showing the levels of WC and FRQ in the indicated strains at different concentrations of QA in LL.