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. Author manuscript; available in PMC: 2013 Sep 7.
Published in final edited form as: Nature. 2013 Feb 17;495(7439):111–115. doi: 10.1038/nature11833

Figure 4.

Figure 4

Codon optimization of the middle region of FRQ impairs FRQ phosphorylation and stabilizes FRQ. (a) Race tube analysis showing the conidiation phenoypes of indicated strains in DD. (b) Western blot showing FRQ expression profile in LL. Three independent samples were shown. (c) Western blots showing FRQ expression profile in DD. Densitometric analysis of the result is shown below. (d) Western blot showing the degradation of FRQ after CHX treatment. The arrow indicates the hyperphosphorylated forms of FRQ after the addition of CHX in the wt-frq strain. Densitometric analyses from four independent experiments are shown below. (e) Western blot showing reduced sensitivity of FRQ from mid-frq to trypsin digestion (2 µg/ml). Densitometric analyses of three independent experiments are shown. Error bars, Standard deviations.