FIG. 4.

PLGA cytotoxicity. (a–d) Cells were fed with PLGA-incubated culture medium to test whether leachable substances from a PLGA scaffold would have cytotoxic effects on the cells. (a, b) No signs of PLGA cytotoxicity was observed in terms of cell morphology; (c, d) Live/Dead^® cell viability assay indicated very few dead cells; (e) No statistical difference was found between control and PLGA group, p=0.05. (f) Visualization of morphology of cells grown on PLGA by fluorescent wheat germ agglutinin staining of cell membranes. SH-SY5Y cells attached, spread, and proliferated on PLGA. Color images available online at www.liebertpub.com/tec