Table 1.
Sequence of primers and their concentration used in multiplex ARMS (MARMS)
| Mutation | Sequence | Used with primer | Primer concentration (nm) | Product size (bp) | |
|---|---|---|---|---|---|
| Normal | Mutant | ||||
| MARMS-A | |||||
| IVS 1–5 (G–C) | CTC CTT AAA CCT GTC TTG TAA CCT TGT TAG a | E | 500 | 500 | 319 |
| Cd 41/42 (–TTCT) | GAG TGG ACA GAT CCC CAA AGG ACT CAA CCT a | E | 50 | 50 | 476 |
| Cd 17 (A–T) | CTC ACC ACC AAC TTC ATC CAC GTT CAG CTA b | E | 50 | 50 | 275 |
| Cd 26 (G–A) | TAA CCT TGA TAC CAA CCT GCC CAG GGC GTT d | E | 50 | 50 | 301 |
| MARMS-B | |||||
| IVS 1–1 (G–T) | TTA AAC CTG TCT TGT AAC CTT GAT ACG AAA a | E | 300 | 600 | 315 |
| Cd 8/9 (+G) | CCT TGC CCC ACA CGG CAG TAA CGG CAC ACC a | E | 500 | 250 | 250 |
| –28 (A–G) | TAA GCA ATA GAT GGC TCT GCC CTG AGT TC b | E | 300 | 180 | 145 |
| Cd 71/72 (+A) | GGT TGT CCA GGT GAG CCA GGC CAT CAG TT b | E | 500 | 140 | 569 |
| MARMS-C | |||||
| IVS 1–1 (G–A) | TTA AAC CTG TCT TGT AAC CTT GAT ACG AAT e | E | 300 | 600 | 315 |
| Cd 43 (G–T) | ATC AGG GAG TGG ACA GAT CCC CAA GGA GTA e | E | 80 | 50 | 482 |
| Cd 16 (–C) | TCA CCA CCA ACT TCA TCC ACG TTC ACG TTC d | E | 80 | 160 | 273 |
| Poly A (A–G) | GGC CTT GAG CAT CTG GAT TCT GCC TAT TAG c | B | 160 | 50 | 393 |
| MARMS-D | |||||
| Cd 19 (A–G) | TGC CGT TAC TGC CCT GTG GGG CAA GGA GAG e | F | 50 | 30 | 173 |
| CAP +1 (A–C) | AAA AGT CAG GGC AGA GCC ATC TAT TGG TTC d | F | 100 | 80 | 281 |
| MARMS-E | |||||
| –88 (C–T) | TCA CTT AGA CCT CAC CCT GTG GAG CCT CAT a | F | 50 | 50 | 369 |
| Initiation Cd | TGT TCA CTA GCA ACC TCA AAC AGA CAG CAG e | F | 100 | 160 | 248 |
| Cd 15 (G–A) | TGA GGA GAA GTC TGC CGT TAC TGC CCA GTA d | F | 60 | 30 | 203 |
| –29 (A–G) | CAG GGA GGG CAG GAG CCA GGG CTG GGT ATG a | F | 50 | 20 | 310 |
| ARMS-F | |||||
| IVS 2–654 (C–T) | GAA TAA CAG TGA TAA TTT CTG GGT TAA CGT a | B | 160 | 400 | 826 |
| Control A | CAA TGT ATC ATG CCT CTT TGC ACC a | 120 | 120 | 861 | |
| Control B | GAG TCA AGG CTG AGA GAT GCA GGA a | 150 | 105 | ||
| Control C | CAA CTT GCT CAA GCA TAC ACT C b | 105 | 105 | 493 | |
| Control D | AAT AAT AGG CAT AGT GAC AAG TGC b | 105 | 105 | ||
| Common E | TGA AGT CCA ACT CCT AAG CCA GTG b | 160 | 160 | ||
| Common F | CAA TAG GCA GAG AGA GTC AGT GCC TAT CA b | 160 | 160 | ||
* Each normal primer was tested with internal control only. Primer sequences (superscripted a–e) in reference were obtained from earlier publications. Sequences tagged “a” were from (16), “b” from (15), “c” from (14), “d” from (17), and “e” were designed during the course of the project. Underlined text identifies mutation, bolded text identifies mismatch.