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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Dev Biol. 2008 Apr 15;320(1):19–29. doi: 10.1016/j.ydbio.2008.03.045

Fig. 2.

Fig. 2

Shh and Lef1 expression is delayed in Wnt1-Cre;Alk5fl/fl teeth. Analysis of Shh and Lef1 expression in wild-type and Wnt1-Cre;Alk5fl/fl maxilla and mandible processes by whole mount in situ hybridization. Arrowheads highlight Shh expression. (A–H) At E11.5 and E12.5, Shh expression is detectable in the maxilla (A, E) and mandible process (C, G) of wild-type embryos, but is only detectable in the maxilla (B, F) and not the mandible (D, H) of Wnt1-Cre;Alk5fl/fl embryos. (I–L) At E13.5, Shh expression is detectable in the maxilla process (I) of wild-type embryos, but it is no longer detectable in the mandible processes (K). In contrast, Shh expression is now clearly detectable in the Wnt1-Cre;Alk5fl/fl maxilla (J) and mandible (L). (M–T) At E12.5 and E13.5, Lef1 expression is detectable in wild-type maxilla (M, Q) and mandible processes (O, S) but not in Wnt1-Cre;Alk5fl/fl littermates (N, R, maxilla; P, T mandible). (U, V) At E14.5, Lef1 expression is visible in both wild-type (U) and mutant (V) tooth germ sections. Open arrowheads indicate the void of expression. Scale bar in panel A=2 mm. Scale bar in panel U=50 μm.