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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Dev Biol. 2008 Apr 15;320(1):19–29. doi: 10.1016/j.ydbio.2008.03.045

Fig. 5.

Fig. 5

Tooth phenotypes are indistinguishable in Wnt1-Cre;Tgfbr2fl/fl;Alk5fl/fl double knockout and Wnt1-Cre;Alk5fl/fl single knockout mice. HE staining of sections from wild-type and Wnt1-Cre;Tgfbr2fl/fl;Alk5fl/fl mice. (A–D) At E12.5, wild-type incisor (A) and molar (C) development has reached the lamina stage. In contrast, epithelium thickening was detectable in the incisor (B) but not the molar (D) region of Wnt1-Cre;Tgfbr2fl/fl; Alk5fl/fl embryos. (E–H) At E15.5, both incisors and molars have developed to the cap stage in wild-type embryos (E, G), whereas they remain in the bud stage in the double knockout embryos (F, H). Arrows indicate locations of tooth germs. Dotted lines outline tooth germs. Scale bar in panel A=50 μm. Scale bar in panel E=100 μm.