Fig. 3.

EIT-specific serum IgG following intramuscular and subcutaneous immunization. Test mice were injected with pCI-neo-EIT (A), pCI-neo-EIT plus protein (B) and rEIT protein (C). Protein was injected using complete and incomplete Freund’s adjuvants, while the construct as DNA vaccine was administered without adjuvant. Immunizations were performed four times within eight weeks. The sera were collected before each injection and assessed for rEIT-specific IgG by ELISA method. Non-immunized mice sera injected with PBS and pCI-neo were used as control.