Figure 6. Assaying the sensitivity of the T295S mutant towards Mg²⁺.

The growth activity of the T295S mutant was monitored in the presence of increasing Co²⁺ concentrations, as well as a fixed Mg²⁺ concentration of (A) 0 μM, (B) 5 μM, (C) 25 μM and (D) 250 μM. A reduction in growth activity upon Co²⁺ concentration increase is indicative of the Co²⁺ transport activity of the TmCorA variant. The wild-type TmCorA (WT corA) and the empty CorA-less pBAD vector (corA-less) were used as positive and negative controls respectively. The results are the means±S.D. of at least three independent experiments.