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. Author manuscript; available in PMC: 2013 Apr 18.
Published in final edited form as: Horm Mol Biol Clin Investig. 2011 Jan 12;3(1):357–366. doi: 10.1515/hmbci.2010.042

Figure 5.

Figure 5

The effect of 5-day estradiol, antiestrogen and letrozole treatment on the growth of MCF-7, LTED, MCF-7Ca and UMB-1Ca cells. MTT cell proliferation assay was performed on (A) MCF-7 cells, (B) LTED cells, (C) MCF-7Ca cells and (D) UMB-1Ca cells. Cells were cultured in phenol red-free charcoal-stripped media for 2 days to deplete cells of steroids. Cells were seeded at a density of 20,000 cells/mL into 24-well plates and allowed to attach overnight. MCF-7 cells were cultured in phenol red-free IMEM for 5 days with vehicle (0.1% ethanol), estradiol, androstenedione, tamoxifen, Faslodex or letrozole. On the day the assay was performed 1 mL of a 1 mg/mL solution of MTT was added to each well and incubated for 3 h. The product was detected using a spectrophotometry at a wavelength of 560 nm. Values are represented as a percentage of the untreated control±SEM (n=3).