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The fluorescent emission spectra are not affected by the protein fusion. mRFP fused to CDKA;1 (A) and free mRFP (B) were transiently expressed in tobacco and the fluorescence emission spectra were measured in the nucleus (marked by a ring) with Olympus FluoView™ FV1000 and Zeiss LSM 710 (C). Scale bars, 30 μm.
