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. 2013 Apr 3;9:10. doi: 10.1186/1746-4811-9-10

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Copyright © 2013 Mylle et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Linear unmixing of spectrally similar fluorophores in tobacco and Arabidopsis cells. (A-D) Tobacco epidermal cells transiently expressing CDKA;1-TagRFP and ER-mCherry marker. (A) Image before unmixing (red channel, 609-619 nm). Fluorophore signals after unmixing: ER-mCherry in the ER strands and nuclear envelope (B) and CDKA;1-TagRFP in the nucleus and the cytoplasm (C). (D) Overlay. (E-H) Arabidopsis roots expressing PIN2-eGFP and AUX1-eYFP. Image before unmixing (green channel, 524-534 nm) (E) and fluorophore signals after unmixing: PIN2-eGFP (G), AUX1-eYFP (F) and overlay (H). Scale bars, 30 μm.