Table 1.
Multi-color Gateway-compatible entry clones
| Fluorescent proteins | Recipient pDONR | Entry clone | att sites |
|---|---|---|---|
| Dendra |
pDONR P4-P1R |
pEN-L4-Dendra-R1 |
attB4-attB1 |
| pDONR221 |
pEN-L1-Dendra-L2 |
attB1-attB2 |
|
| pDONR221 |
pEN-L1-Dendra*-L2 |
attB1-attB2 |
|
| pDONR P2R-P3 |
pEN-R2-Dendra*-L3 |
attB2-attB3 |
|
| Venus |
pDONR P4-P1R |
pEN-L4-Venus-R1 |
attB1-attB2 |
| pDONR221 |
pEN-L1- Venus -L2 |
attB1-attB2 |
|
| pDONR221 |
pEN-L1- Venus *-L2 |
attB1-attB2 |
|
| pDONR P2R-P3 |
pEN-R2- Venus *-L3 |
attB2-attB3 |
|
| mCherry |
pDONR P4-P1R |
pEN-L4-mCherry-R1 |
attB4-attB1 |
| pDONR221 |
pEN-L1- mCherry -L2 |
attB1-attB2 |
|
| pDONR221 |
pEN-L1- mCherry *-L2 |
attB1-attB2 |
|
| pDONR P2R-P3 |
pEN-R2- mCherry *-L3 |
attB2-attB3 |
|
| TagRFP |
pDONR P4-P1R |
pEN-L4-TagRFP-R1 |
attB4-attB1 |
| pDONR221 |
pEN-L1- TagRFP -L2 |
attB1-attB2 |
|
| pDONR221 |
pEN-L1- TagRFP *-L2 |
attB1-attB2 |
|
| pDONR P2R-P3 |
pEN-R2- TagRFP *-L3 |
attB2-attB3 |
|
| Cerulean | pDONR P4-P1R |
pEN-L4-Cerulean-R1 |
attB4-attB1 |
| pDONR221 |
pEN-L1- Cerulean -L2 |
attB1-attB2 |
|
| pDONR221 |
pEN-L1- Cerulean *-L2 |
attB1-attB2 |
|
| pDONR P2R-P3 | pEN-R2- Cerulean *-L3 | attB2-attB3 |
* indicates STOP codons.