Figure 1).

Genotyping of Acinetobacter species isolates. Multiplex PCR for the gyrB gene identified clinical isolates as Acinetobacter baumannii, Acinetobacter pittii or Acinetobacter nosocomialis. Presence of a 294 bp band indicates A nosocomialis, and a 193 bp band indicates A pittii. Presence of two bands at 294 bp and 490 bp indicates A baumannii. Lane 1 Acinetobacter species AB004, Lane 2 Acinetobacter species AB005, Lane 3 Acinetobacter species AB006, Lane 4 Acinetobacter species AB007, Lane 5 Acinetobacter species AB008, Lane 6 Acinetobacter species AB009, Lane 7 Acinetobacter species AB010, Lane 8 Acinetobacter species AB011, Lane 9 Acinetobacter species AB012, Lane 10 Acinetobacter species AB013, Lane 11 Acinetobacter species AB014, Lane 12 Acinetobacter species AB027, Lane 13 Acinetobacter species AB028, Lane 14 Acinetobacter species AB029, Lane 15 Acinetobacter species AB030, Lane 16 Acinetobacter species AB031, Lane 17 A baumannii ATCC 19606. M Molecular weight marker; N No-template control