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. 2013 Apr 18;9(4):e1003387. doi: 10.1371/journal.pgen.1003387

Figure 1. TIP48 and H2A.Z are required for CCND1 activation by estradiol.

Figure 1

A) MCF-7 cells were cultivated 3 days in steroid stripped (white) medium and then induced by E2 10−7 M for 6 h. CCND1, H2AFZ mRNA was quantified by real-time RT-PCR. The mean and SD from three independent experiments are shown. (*) indicates a p value<0.05 (Student t-test). B) MCF-7 H2A.Z ChIP on chip signal detected around the CCND1 gene on human chromosome 11 loaded from [32] on UCSC. C, D) H2A.Z (C) and TIP48 (D) occupancy at CCND1 TSS and enh2 before and after 30 min and 60 min of E2 10−7 M treatment analysed by ChIP. The values of ChIP efficiencies are given in % of input with s.e.m indicated (n = 3). Positions of DNAseI hypersensitive sites and primers used for ChIP are indicated in C).