Skip to main content
. 2013 Apr 18;9(4):e1003387. doi: 10.1371/journal.pgen.1003387

Figure 2. TIP48 regulates estrogen-activated transcription via H2A.Z release.

Figure 2

A, B) MCF-7 cells were cultivated 3 days in steroid free medium, transfected with, scramble (ctrl) or TIP48 siRNA for 72 h and induced by E2 10−7 M for 30 min. Association of H2A.Z (A) and histone H3 (B) with TSS and enh2 sites was analyzed by ChIP and shown as % input (n = 2). C) MCF-7 cells were cultivated 3 days in steroid free medium and transfected at day 0 with a Scramble (Ctrl), a H2A.Z siRNA or a TIP48 siRNA for 72 h and then induced by E2 10−7 M for 6 h. CCND1 mRNA levels were determined by qRT-PCR. Control (Ctrl) without E2 treatment was set to 1. The mean and SD from three independent experiments are shown. (*) indicates a p value<0.05.