Figure 3. TIP48 is required for ERα binding during E2 activated transcription.

MCF-7 cells were cultivated 3 days in steroid free medium, transfected with scramble (ctrl) or TIP48 siRNA for 72 h and induced by E2 10⁻⁷ M for 30 min. A) ChIP analysis of ERα occupancy at the CCND1 TSS and enh2 sites. Results are shown as % input (n = 2). B) qRT-PCR quantification of ESR1 mRNA expression relative to RPLO. The mean and SD from three independent experiments are shown. (**) indicates a p value<0.01.