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. 2013 Apr 18;9(4):e1003387. doi: 10.1371/journal.pgen.1003387

Figure 4. TIP48 promotes TIP60 binding and CCND1 transcription activation.

Figure 4

MCF-7 cells were cultivated 3 days in steroid free medium and induced by E2 10−7 M for 6 h. A) Cells were transfected with scramble (-) or TIP48 siRNA for 72 h and induced by E2 10−7 M for 30 min. TIP60 binding to the CCND1 promoter was analyzed by ChIP and shown as percent of input (n = 2). B) After 24 h in steroid free medium, MCF-7 cells were transfected with a mock vector or a vector expressing TIP60 for 48 h. CCND1 mRNA expression was analyzed by qRT-PCR. The mean and SD from three independent experiments are shown. (*) indicates a p value<0.05. C) MCF-7 cells were first transfected with scramble (-) or TIP48 siRNA as in (A). After 24 h, same cells were transfected with a mock vector or a vector expressing TIP60 for 48 h as in (B). CCND1 gene expression was analyzed by qRT-PCR after 30 min of E2 10−7 M induction.