Figure 6. Release of CCND1 gene looping requires TIP48.

A) Schematic representation of the CCND1 locus on h.s. chromosome 11 (adapted from the UCSS genome browser http://genome.ucsc.edu/cgi-bin/hgGateway). DNAseI hypersensitive sites [7], Csp6I restriction sites and fragments assayed by 3C are represented. B) Quantitative PCR amplification of ligation events in a 3C assay in MCF-7 cells treated or not with E2 10⁻⁷ M for 45 min and transfected 72 h before with scramble (ctrl) or TIP48 siRNA. Crosslinked DNA was digested with the Csp6I restriction enzyme and ligated. qRT-PCR was performed with primers designed for the 4 possible ligation events (only one is shown).