Table 3. Amino acids at key tuning sites for RH1 [21], [25] and LWS [36].

Branch Node Numbers (Ancestor to Descendant)	Branch Name	RH1		LWS
		Amino Acid Changes at Key Tuning Sites(83 292 299)	λmax (nm) Change on Branch	Amino Acid Changes at Key Tuning Sites(180 197 277 285 308)	λmax (nm) Change on Branch
26 to 27	Stem Cetacea	DAS to NSS	501 to 484	No changes	No change
27 to 28	Stem Mysticeti	No change	No change	AHYTA to AHYTS	552 to 522–531
28 to 29	Stem Balaenidae	NSS to NAS	484 to 493	No change	No change
30 to 4	Caperea	NSS to NSA	484 to 479	No change	No change
33 to 6	Eschrichtius	NSS to NAS	484 to 493	No change	No change (pseudogene)
34 to 7	Megaptera	NSS to DSS	484 to 492	No change	No change (pseudogene)
35 to 36	Stem Physeteroidea	NSS to NSA	484 to 479	No change	No change
38 to 39	Stem Ziphiidae	NSS to NSA	484 to 479	No change	No change
42 to 43	Stem Iniidae + Pontoporiidae	NSS to NAS	484 to 493	No change	No change
43 to 17	Pontoporia	NAS to DAS	493 to 501	No change	No change
43 to 18	Inia	NAS to NAT*	493 to ?	AHYTA to AHYTP*	552 to ?
44 to 45	Stem Monodontidae + Phocoenidae	NSS to DSS	484 to 492	No change	No change
45 to 22	Delphinapterus	DSS to DAS	492 to 501	No change	No change
42 to 44	Stem Delphinoidea	No change	No change	AHYTA to AHYTS	552 to 522–531

Node numbers correspond to Figure 1. RH1 λ_max values are based on Fasick et al. [21] for DAS and Bischoff et al. [25] for all other amino acid combinations. LWS λ_max values are based on Fasick et al. [21].

^* = λ_max value unknown.