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. 2013 Apr 18;9(4):e1003305. doi: 10.1371/journal.ppat.1003305

Figure 4. The formation of mating projections and a-α mating are mediated by the Cph1 transcription factor in opaque cells.

Figure 4

(A) Deletion of STE2 or CPH1 abolished elongated projection formation and mating by C. albicans opaque cells. In contrast, TEC1 is not required for pheromone responses in the opaque state. Numbers are the mean ± SD from two independent experiments with three replicates. Images of (B) mating projections formed in MTL a opaque cells responding to α pheromone, and (C) zygotes produced from mating between MTL a and MTLα opaque cells. Scale bar: 5 µm. Each data point is the mean ± SD from two independent experiments with at least three replicates. (WT: CAY1477; Δste2/Δste2: CAY1478; Δtec1/Δtec1: CAY2689; TEC1 AB: CAY2775; Δcph1/Δcph1: CAY2947; CPH1 AB: CAY3046; MTLα: DSY211).