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. 2013 Apr 18;9(4):e1003305. doi: 10.1371/journal.ppat.1003305

Figure 6. Examination of six downstream targets of Cph1 and Tec1 reveals a general role for Hgc1 in biofilm formation.

Figure 6

(A) Heatmap showing expression of six target genes that are regulated by Cph1 and Tec1 during pheromone treatment of white cells. (B) Pheromone-stimulated biofilm formation in mutants lacking each of the six candidate genes. Top panel, images of white cells adhering to plastic. Bottom panel, quantification of the number of adherent cells. Light blue bars on graphs, no pheromone added. Dark blue bars, MFα pheromone present. (C) Analysis of the six candidate genes in a conventional biofilm assay on silicone squares. Hgc1 and Tec1 are both necessary for conventional biofilm formation. Values are the mean ± SD from two independent experiments with at least three replicates. “#” represents P<0.01 and “*” represents P<0.001 for the difference with the wildtype strain. The complemented HGC1 strain (HGC AB) showed a significant increase in biofilm formation compared to the hgc1 mutant. (WT: CAY716; Δste2/Δste2: CAY1234; Δorf19.7167/Δorf19.7167: CAY3445; Δorf19.7170/Δorf19.7170: CAY3447; Δorf19.7305/Δorf19.7305: CAY3693; Δpbr1/Δpbr1: CAY3689; Δcfl11/Δcfl11: CAY3687; Δhgc1/Δhgc1: CAY3465; HGC1 AB: CAY3702).